A computerized Infusion Pump for control oftissue tracer concentration during Positron Emission Tomography in vivo Pharmacokinetic/Pharmacodynamic measurements.

A computer controlled infusion pump (UIPump) for regulation of target tissue concentration of radioactive compounds was developed for use in biological research and tracer development for PET.

Methods: Based on observed tissue or plasma kinetics after a bolus injection of the tracer an algorithm calculates the infusion needed to obtain a specified target kinetic curve.

A computer feeds this infusion scheme into an infusion pump connected to an animal via a venous catheter. The concept was validated using [11C]Flumazenil administrated to Sprague-Dawley rats where the whole brain distribution and kinetic of the tracer was measured over time using a microPET-scanner.

The accuracy and precision of the system was assessed by producing steady-state levels of the tracer and by mimicking kinetics after oral administration.

Results: Various kinetic profiles could be generated, including rapid achievement of constant levels, or step-wise increased levels.

The resulting tissue curves had low deviation from the target curves according to the specified criteria: AUC (%): 4.2 +/-2.8, Maximal deviation (%): 13.6 +/-5.0 and R2: 0.95 +/-0.02.

Conclusions: The UIPump-system is suitable for use in PET-research for assessment of PK/PD properties by simulation of different tracer tissue kinetics in vivo.

Author: Olof Eriksson, Andreas Wallberg, Stina Syvanen, Raymond Josephsson, Bengt Langstrom and Mats Bergstrom
Credits/Source: BMC Medical Physics 2008, 8:2

Published on: 2008-05-30

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