In vitro behaviour of endothelial cells on a titanium surface
Endothelial cells play an important role in the delivery of cells to the inflammation site, chemotaxis, cell adhesion and extravasation. Implantation of a foreign material into the human body determines inflammatory and repair reactions, involving different cell types with a plethora of released chemical mediators.
The evaluation of the interaction of endothelial cells and implanted materials must take into account other parameters in addition to the analysis of maintenance of cell viability.
Methods: In the present investigation, we examined the behavior of human umbilical vein endothelial cells (HUVECs) harvested on titanium (Ti), using histological and immunohistochemical methods.
The cells, after two passages, were seeded in a standard density on commercially plate-shaped titanium pieces, and maintained for 1, 7 or 14 days.
Results: After 14 days, we could observe a confluent monolayer of endothelial cells (ECs) on the titanium surface.
Upon one-day Ti/cell contact the expression of fibronectin was predominantly cytoplasmatic and stronger than on the control surface. It was observed strong and uniform cell expression along the time of alpha5beta1 integrin on the cells in contact with titanium.
Conclusions: The attachment of ECs on titanium was found to be related to cellular-derived fibronectin and the binding to its specific receptor, the 51 integrin. It was observed that titanium effectively serves as a suitable substrate for endothelial cell attachment, growth and proliferation.
However, upon a 7-day contact with Ti, the Weibel-Palade bodies appeared to be not fully processed and exhibited an anomalous morphology, with corresponding alterations of PECAM-1 localization.
Author: Ana C Breithaupt-Faloppa, Wothan Tavares de Lima, Ricardo M Oliveira-Filho and Johannes Kleinheinz
Credits/Source: Head &Face Medicine 2008, 4:14
Published on: 2008-07-23
Copyright by the authors listed above - made available via BioMedCentral (Open Access). Please
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