Extensive dynamics of Plasmodium falciparum densities, stages and genotyping profiles

Individuals living in areas of high malaria transmission often have different Plasmodium falciparum clones detected in the peripheral blood over time. The aim of this study was to assess the dynamics of asymptomatic P.

falciparum infections in a few hours intervals.

Methods: Capillary blood samples were collected 6-hourly during 5 days from asymptomatic children in a highly endemic area in Tanzania. Parasite densities and maturation stages were investigated by light microscopy.

Types and number of clones were analysed by PCR based genotyping of the polymorphic merozoite surface proteins 1 and 2 genes.

Results: Parasite densities and maturation stages fluctuated 48 hourly with a gradual shift into more mature forms.

Various genotyping patterns were observed in repeated samples over five days with only few samples with identical profiles. Up to six alleles differed in samples collected 6 hours apart in the same individual.

Conclusion: This detailed assessment highlights the extensive within-host dynamics of P. falciparum populations and the limitations of single blood samples to determine parasite densities, stages and genotyping profiles in a malaria infected individual.

Author: Anna Farnert, Marianne Lebbad, Lea Faraja and Ingegerd Rooth
Credits/Source: Malaria Journal 2008, 7:241

Published on: 2008-11-21

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