Human parainfluenza virus type 2 (HPIV2) induced host ADAM8 expression in human salivary adenocarcinoma cell line (HSY) during cell fusion
The aim of the study was to investigate expression of ADAMs (A Disintegrin and A Metalloproteinase) of host cell origin during cell-cell fusion induced by human parainfluenza virus type 2 (HPIV2).
Results: Induction of host cell ADAM9 was observed in GMK cells, but the applicability of this model was restricted by lack of cross-reactivity of the anti-human ADAM8 antibodies with the corresponding green monkey antigens.
HSG cells were not susceptible to HPIV2 virus infection. In contrast, in human parotid gland HSY cells, a natural host cell for paramyxoviruses, HPIV2 induced ADAM8 expression.
ADAM8 staining increased dramatically over time from 7.9+/-3 % at zero hour to 99.2+/-0.8 % at 72 hours (p=0.0001). Without HPIV2 the corresponding percentages were only 7.7 % and 8.8 %.
Moreover, ADAM8 positive cells formed bi- (16.2 %) and multinuclear cells (3.5 %) on day one and the corresponding percentages on day three were 15.6 % for binuclear and 57.2 % for multinuclear cells.
Conclusions: ADAM8, well recognized for participation in cell-to-cell fusion especially in osteoclast formation, is upregulated upon formation of multinuclear giant cells after HPIV2 induction in HSYcells.
The virus-HSY cell system provides a novel experimental model for study of the molecular mechanism of cell fusion events.
Author: Guo-Feng Ma, Simo Miettinen, Pauliina Porola, Klaus Hedman, Jari Salo and Yrjo T Konttinen
Credits/Source: BMC Microbiology 2009, 9:55
Published on: 2009-03-16
Copyright by the authors listed above - made available via BioMedCentral (Open Access). Please
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