A comparison between protein profiles of B lymphocyte subpopulations and mantle cell lymphoma cells

B-cell lymphomas are thought to reflect different stages of B-cell maturation. Based on cytogenetics and molecular markers, mantle cell lymphoma (MCL) is presumed to derive predominantly from naive, pre-germinal centre (pre-GC) B lymphocytes.

The aim of this study was to develop a method to investigate the similarity between MCL and different B-cell compartments on a protein expression level.

Methods: Subpopulations of B cells representing the germinal centre (GC), the pre-GC mantle zone and the post-GC marginal zone were isolated from tonsils using automated magnetic cell sorting (AutoMACS) of cells based on their expression of CD27 and IgD. Protein profiling of the B cell subsets, of cell lines representing different lymphomas and of primary mantle cell lymphoma (MCL) samples was performed using top-down proteomics profiling by surface-enhanced laser detection/ ionization time-of-flight mass spectrometry (SELDI-TOF-MS).

Results: Quantitative MS data of significant protein peaks (p-value <0.05) separating the three B-cell subpopulations were generated.

Together, hierarchical clustering and principal component analysis (PCA) showed that the primary MCL samples clustered together with the pre- and post-GC subpopulations. Both primary MCL cells and MCL cell lines were clearly separated from the B cells representing the GC compartment.

Conclusion: AutoMACS sorting generates sufficient purity to enable a comparison between protein profiles of B cell subpopulations and malignant B lymphocytes applying SELDI-TOF-MS.

Further validation with an increased number of patient samples and identification of differentially expressed proteins would enable a search for possible treatment targets that are expressed during the early development of MCL.

Author: Henrik StranneheimLukas OrreJanne LehtioJenny Flygare
Credits/Source: Proteome Science 2009, 7:43

Published on: 2009-11-23

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