Relationship between the expression of hTERT and EYA4 mRNA in peripheral blood mononuclear cells with the progressive stages of carcinogenesis of the esophagus
ObjectiveTo establish a relationship between esophageal squamous cell diseases and the expression of human telomerase reverse transcriptase (hTERT) and Eyes absent 4 (EYA4) mRNA in peripheral blood mononuclear cells.
Methods: Subjects were 50 with esophageal squamous cell carcinoma (ESCC), 50 with dysplasia (ESCD), 50 patients with basal cell hyperplasia (BCH) and 50 controls. All subjects were residents of Feicheng County, Shandong Province, China diagnosed by histopathology.
Expression of hTERT and EYA4 mRNA in peripheral blood was determined by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR).
Results: The hTERT and EYA4 mRNA positive expression increased according to disease severity. At the cut-off value of [greater than or equal to]0.2, the positive expression rates of EYA4 were 15% for controls, 20.0% for BCH, 26% FOR ESCD and 52% for ESCC, respectively.
At the cut-off value of [greater than or equal to]0.8, the positive expression rates of hTERT in the four groups were 24%, 30.0%, 52% and 80%, respectively. Using a positive value of 0.47 for EYA4, the testing sensitivities in the ESCD and ESCC groups were 4% and 16%, respectively, and the testing specificity increased to 100%.
Using a positive value of 1.0 for hTERT, the testing sensitivities in the ESCD and ESCC groups were 48% and 60%, respectively, and the testing specificity increased to 72%. The testing sensitivities in the predicting ESCD and ESCC in the discriminant model including EYA4 and hTERT and the five traditional risk factors sex, age, smoking, alcohol drinking, and family history of esophageal cancer were 70%, and 76%, and testing specificities were 76% and 88% respectively.
However, the testing sensitivities and specificities in the predicting ESCD and ESCC in the model only including the above five traditional risk factors were lower than that in the former case.
Conclusion: EYA4 and hTERT mRNA expression increased with the severity of esophageal pathological changes and may be useful for identifying high-risk endoscopy candidates or for monitoring changes in premalignant esophageal lesions.
Author: Hao LiTao DiaoZhi ZhouFang YangQing MaQing Li
Credits/Source: Journal of Experimental &Clinical Cancer Research 2009, 28:145
Published on: 2009-11-25
Copyright by the authors listed above - made available via BioMedCentral (Open Access). Please
make sure to read our disclaimer prior to contacting 7thSpace Interactive. To contact our editors, visit our online helpdesk. If you wish submit your own press release, click here.
Social Bookmarking
RETWEET This! | Digg this! | Post to del.icio.us | Post to Furl | Add to Netscape | Add to Yahoo! | Rojo
|
|
