Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection

Tissue resident mesenchymal stem cells (MSCs) are multipotent, self-renewing cells known for their differentiation potential into cells of mesenchymal lineage. The ability of single cell clones isolated from adipose tissue resident MSCs (ASCs) to differentiate into cells of hematopoietic lineage has been previously demonstrated.

In the present study, we investigated if the hematopoietic differentiated (HD) cells derived from ASCs could productively be infected with HIV-1.

Results: HD cells were generated by differentiating clonally expanded cultures of adherent subsets of ASCs (CD90+, CD105+, CD45-, and CD34-). Transcriptome analysis revealed that HD cells acquire a number of elements that increase their susceptibility for HIV-1 infection, including HIV-1 receptor/co-receptor and other key cellular cofactors.

HIV-1 infected HD cells (HD-HIV) showed elevated p24 protein and gag and tat gene expression, implying a high and productive infection. HD-HIV cells showed decreased CD4, but significant increase in the expression of CCR5, CXCR4, Nef-associated factor HCK, and Vpu-associated factor BTRC.

HIV-1 restricting factors like APOBEC3F and TRIM5 also showed up regulation. HIV-1 infection increased apoptosis and cell cycle regulatory genes in HD cells.

Although undifferentiated ASCs failed to show productive infection, HIV-1 exposure increased the expression of several hematopoietic lineage associated genes such as c-Kit, MMD2, and IL-10.

Conclusions: Considering the presence of profuse amounts of ASCs in different tissues, these findings suggest the possible role that could be played by HD cells derived from ASCs in HIV-1 infection. The undifferentiated ASCs were non-permissive to HIV-1 infection; however, HIV-1 exposure increased the expression of some hematopoietic lineage related genes.

The findings relate the importance of ASCs in HIV-1 research and facilitate the understanding of the disease process and management strategies.

Author: Timo Nazari-ShaftiEva FreisingerUpal RoyChristine BulotChristiane SenstCharles DupinAbigail ChaffinSudesh SrivastavaDebasis MondalEckhard AltReza Izadpanah
Credits/Source: Retrovirology 2011, 8:3

Published on: 2011-01-12

Copyright by the authors listed above - made available via BioMedCentral (Open Access). Please make sure to read our disclaimer prior to contacting 7thSpace Interactive. To contact our editors, visit our online helpdesk. If you wish submit your own press release, click here.

Comments Page 1 of 1

stdsingle

Posted 497 days ago

Living with HIV/AIDS is not so terrible if we know how to control it.. Maybe you can check more HIV/AIDS blogs and inspirational stories at pozmingle,com. The STD counselor on the site will give you some advice about how to control it.

pozguy

Posted 498 days ago

My friend told me a report from CNN said that the world's largest HIV dating and support site HIVromances . c o m has more than 1,100,000 members. Also, more than 80% of members on the site are good looking and sexy.
The report also indicated that HIV rates soar worldwide and CDC had granted CSU $1.9M for HIV education to lower the rates of HIV transmission.

<1>

+ Add New Comment

Custom Search