BMP-2 gene-fibronectin-apatite composite layer enhances bone formation.
Safe and efficient gene transfer systems are needed for tissue engineering. We have developed an apatite composite layer including the bone morphogenetic protein-2 (BMP-2) gene and fibronectin (FB), and we evaluated its ability to induce bone formation.
Methods:
An apatite composite layer was evaluated to determine the efficiency of gene transfer to cells cultured on it.
Cells were cultured on a composite layer including the BMP-2 gene and FB, and BMP-2 gene expression, BMP-2 protein concentrations, alkaline phosphatase (ALP) activity, and osteocalcin (OC) concentrations were measured. A bone defect on the cranium of rats was treated with hydroxyapatite (HAP)-coated ceramic buttons with the apatite composite layer including the BMP-2 gene and FB (HAP-BMP-FB).
The tissue concentration of BMP-2, bone formation, and the expression levels of the BMP-2, ALP, and OC genes were all quantified.
Results:
The apatite composite layer provided more efficient gene transfer for the cultured cells than an apatite composite layer without FB. The BMP-2 concentration was approximately 100~600 pg/mL in the cell-culture medium.
Culturing the cells on the apatite composite layer for 27 days increased ALP activity and OC concentrations. In animal experiments, the tissue concentrations of BMP-2 were over 100 pg/mg in the HAP-BMP-FB group and approximately 50 pg/mg in the control groups.
Eight weeks later, bone formation was more enhanced in the HAP-BMP-FB group than in the control groups. In the tissues surrounding the HAP button, the gene expression levels of ALP and OC increased.
Conclusion:
The BMP-2 gene-FB-apatite composite layer might be useful for bone engineering.
Author: Wei ZhangHideo TsurushimaAyako OyaneYushin YazakiYu SogoAtsuo ItoAkira Matsumura
Credits/Source: Journal of Biomedical Science 2011, 18:62
Published on: 2011-08-23
Copyright by the authors listed above - made available via BioMedCentral (Open Access). Please
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