Bone marrow stromal cell antigen 2 (BST-2) restricts mouse mammary tumor virus (MMTV) replication in vivo
Bone marrow stromal cell antigen 2 (BST-2) is a cellular factor that restricts the egress of viruses such as human immunodeficiency virus (HIV-1) from the surface of infected cells, preventing infection of new cells. BST-2 is variably expressed in most cell types and its expression is enhanced by cytokines such as type I interferon alpha (IFN-alpha).
In this present study, we used the beta-retrovirus, mouse mammary tumor virus (MMTV) as a model to examine the role of mouse BST-2 in host infection in vivo.
Results:
By using RNA interference, we show that loss of BST-2 enhances MMTV replication in cultured mammary tumor cells and in vivo. In cultured cells, BST-2 inhibits virus accumulation in the culture medium, and co-localizes at the cell surface with virus structural proteins.
Furthermore, both scanning electron micrograph (SEM) and Transmission electron micrograph (TEM) show that MMTV accumulates on the surface of IFN alpha-stimulated cells.
Conclusions:
Our data provide evidence that BST-2 restricts MMTV release from naturally infected cells and that BST-2 is an antiviral factor in vivo.Running Title: BST-2 restriction of virus replication in vivoKeywordsBST-2, Tetherin, Interferon alpha, MMTV, In vivo, SEM, TEM
Author: Philip H JonesHarshini V MehtaMartina MaricRichard J RollerChioma M Okeoma
Credits/Source: Retrovirology 2012, 9:10
Published on: 2012-01-27
Copyright by the authors listed above - made available via BioMedCentral (Open Access). Please
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