Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis
Haemophilus parasuis is the etiological agent of Glasser's disease in pigs. Currently, the molecular basis of this infection is largely unknown.
The innate immune response is the first line of defense against the infectious disease. Systematical analysis on host innate immune response to the infection is important for understanding the pathogenesis of the infectious microorganisms.
Results:
A total of 428 differentially expressed genes were identified in the porcine alveolar macrophages 6 days after H.parasuis infection.
These genes were principally related to inflammatory response, immune response, microtubule polymerization, regulation of transcript and signal transduction. Through the pathway analysis, the significant pathways mainly concerned with cell adhesion molecules, cytokine-cytokine receptor interaction, complement and coagulation cascades, toll-like receptor signaling pathway, MAPK signaling pathway, suggesting that the host took different strategies to activate immune and inflammatory response upon H.parasuis infection.
The global interactions network and two subnetworks of the proteins encoded by DE genes were analyzed by using STRING. Further immunostimulation analysis indicated that mRNA levels of S100 calcium-binding protein A4 (S100A4) and S100 calcium-binding protein A6 (S100A6) in porcine PK-15 cells increased within 48h and were sustained after administration of lipopolysaccharide (LPS) and Poly (I:C) respectively.
The s100a4 and s100a6 genes were found to be up-regulated significantly in lungs, spleen and lymph nodes in H.parasuis infected pigs. We firstly cloned and sequenced the porcine coronin1a gene.
Phylogenetic analysis showed that poCORONIN 1A belonged to the group containing the Bos taurus sequence. Structural analysis indicated that the poCORONIN 1A contained putative domains of Trp-Asp (WD) repeats signature, Trp-Asp (WD) repeats profile and Trp-Asp (WD) repeats circular profile at the N-terminus.
Conclusions:
Our present study is the first one focusing on the response of porcine alveolar macrophages to H.parasuis.
Our data demonstrate a series of genes are activated upon H.parasuis infection. The observed gene expression profile could help screening thepotential host agents for reducing the prevalence of H.parasuis and further understanding the molecular pathogenesis associated with H.parasuis infection in pigs.
Author: Yang WangChong LiuYing FangXiaoli LiuWentao LiShuqing LiuYingyu LiuYuxi LiuCatherine CharreyreJean-Christophe AudonnetPin ChenQigai He
Credits/Source: BMC Genomics 2012, 13:68
Published on: 2012-02-13
Copyright by the authors listed above - made available via BioMedCentral (Open Access). Please
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