Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP in heterologous host
Holins are a group of phage-encoded membrane proteins that control access of phage-encoded endolysins to the peptidoglycan, and thereby trigger the lysis process at a precise time point as the 'lysis clock'. SMP is an isolated and characterized Streptococcus suis lytic phage.
The aims of this study were to determine the holin gene, HolSMP, in the genome of SMP, and characterized the function of holin, HolSMP, in phage infection.
HolSMP was predicted to encode a small membrane protein with three hydrophobic transmembrane helices. During SMP infections, HolSMP was transcribed as a late gene and HolSMP accumulated harmlessly in the cell membrane before host cell lysis.
Expression of HolSMP in Escherichia coli induced an increase in cytoplasmic membrane permeability, an inhibition of host cell growth and significant cell lysis in the presence of LySMP, the endolysin of phage SMP. HolSMP was prematurely triggered by the addition of energy poison to the medium.
HolSMP complemented the defective lambda S allele in a non-suppressing Escherichia coli strain to produce phage plaques.
Our results suggest that HolSMP is the holin protein of phage SMP and a two-step lysis system exists in SMP.
Author: Yibo ShiYaxian YanWenhui JiBin DuXiangpeng MengHengan WangJianhe Sun
Credits/Source: Virology Journal 2012, 9:70
Published on: 2012-03-22
Copyright by the authors listed above - made available via BioMedCentral
(Open Access). Please
make sure to read our disclaimer
prior to contacting 7thSpace Interactive. To contact our editors, visit our online helpdesk
. If you wish submit your own press release, click here