The transcriptional regulator gene E2 of the Human Papillomavirus (HPV) 16 influences the radiosensitivity of cervical keratinocytes

Clinical studies have demonstrated that HPV induced tumors constitute a specific subclass of cancer with a better response to radiation treatment. The purpose of this study was to investigate meaning of viral E2-gene for radiosensitivity.

Methods: W12 cells contain episomal HPV 16 genomes, whereas S12 cells, which derive from the W12 line, contain HPV DNA as integrated copies.

Clonogenic survival was analyzed using 96-well in vitro test. Using flow cytometry cell cycle analyses were performed.

Expression of pRb and p53 were analyzed using intracellular staining.

Results: W12 cells (intact E2 gene) showed a lower survival fraction than S12 cells. W12 cells developed a G2/M block 24 h after irradiation with 2 Gy whereas S12 showed no G2/M bloc.

After irradiation S12 cells developed polyploidy and pRb-positive cells decreased. W12 cells showed no change of pRb-positive cells.

Conclusions: Depending on E2 gene status differences in cell cycle regulation might cause radioresistance.

The E2/E7/pRb pathway seems to influence HPV-induced radiosensitivity. Our experiments demonstrated an effect of HPV on radiosensitivity of cervical keratinocytes via viral transcription regulator E2 pathway.

Author: Katja LindelStefan RiekenSigrid DaffingerKlaus J WeberEthel-Michele de VilliersJ├╝rgen Debus
Credits/Source: Radiation Oncology 2012, 7:187

Published on: 2012-11-07

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