Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells

Teeth are vital not only for a good smile, but also good health. Yet, we lose teeth regularly due to accidents or diseases.

An ideal solution to this problem is to regenerate teeth with patients'own cells. Here we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells (ifhU-iPSCs).

Results: We first differentiated ifhU-iPSCs to epithelial sheets, which were then recombined with E14.5 mouse dental mesenchymes.

Tooth-like structures were recovered from these recombinants in 3 weeks with success rate up to 30% for 8 different iPSC lines, comparable to H1 hESC. We further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures, possessing physical properties such as elastic modulus and hardness found in the regular human tooth.

Conclusion: Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies.

Author: Jinglei CaiYanmei ZhangPengfei LiuShubin ChenXuan WuYuhua SunAng LiKe HuangRongping LuoLihui WangYing LiuTing ZhouShicheng WeiGuangjin PanDuanqing Pei
Credits/Source: Cell Regeneration 2013, 2:6

Published on: 2013-07-30

News Provider: 7thSpace Interactive / EUPB Press Office

Copyright by the authors listed above - made available via BioMedCentral (Open Access). Please make sure to read our disclaimer prior to contacting 7thSpace Interactive. To contact our editors, visit our online helpdesk. If you wish submit your own press release, click here.

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