While it remains a far way off, scientists hope one day to be able to produce hearts suitable for transplant into humans as well as patches to regenerate defective hearts.
The heart produced by researchers at Tel Aviv University is about the size of a rabbit's.
It marked "the first time anyone anywhere has successfully engineered and printed an entire heart replete with cells, blood vessels, ventricles and chambers," said Tal Dvir, who led the project.
"People have managed to 3D-print the structure of a heart in the past, but not with cells or with blood vessels," he said.
But the scientists said many challenges remain before fully working 3D-printed hearts would be available for transplant into patients.
Journalists were shown the printed heart - about the size of a cherry - immersed in liquid, at Tel Aviv University on Monday as the researchers announced their findings, published in the peer-reviewed journal Advanced Science.
Researchers must now teach the printed hearts "to behave" like real ones. The cells are currently able to contract, but do not yet have the ability to pump.
Then they plan to transplant them into animal models, hopefully in about a year, said Dvir.
Their research involved taking a biopsy of fatty tissue from patients that was used in the development of the "ink" for the 3D print.
First, patient-specific cardiac patches were created followed by the entire heart, the statement said.
Using the patient's own tissue was important to eliminate the risk of an implant provoking an immune response and being rejected, Dvir said.
"The biocompatibility of engineered materials is crucial to eliminating the risk of implant rejection, which jeopardises the success of such treatments," said Dvir.
Challenges that remain include how to expand the cells to have enough tissue to recreate a human-sized heart, he said.
Current 3D printers are also limited by the size of their resolution and another challenge will be figuring out how to print all small blood vessels. (AFP)